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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene is observed in several tumors, which ends up in chromosome instability, and therefore might add to tumorigenesis. This protein was observed to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial role in endorsing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have already been explained. Two further splice variants were being documented but specific nucleotide sequence information just isn't nevertheless available.

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Blocking peptides are peptides that bind specially towards the focus on antibody and block antibody binding. These peptide ordinarily includes the epitope identified by the antibody.

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This tutorial outlines even more achievable optimization of cellular and immunoassay parameters to be certain the absolute best final results are...

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This guidebook outlines even more attainable optimization of mobile and immunoassay parameters to guarantee the absolute best benefits are...

Antibodies certain to the blocking peptide no more bind on the epitope about the focus on protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is precise.

Focus on Data Cyclin E belongs on the very conserved cyclin family members, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which lead towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S transition. This protein accumulates in the G1-S section boundary and is also degraded as cells development by way of S phase.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by equally antibodies...

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